Major surface antigen gene of a human malaria parasite cloned and expressed in bacteria

ABSTRACT The late blood stages of the human malaria parasite, _Plasmodium falciparum_, carry a major surface antigen, p190, of molecular weight (_M_r) 190,000 (ref. 1). This antigenically

variable protein1–3 is actively processed, first as the parasite matures and again when it is released into the blood stream and invades a new erythrocyte to initiate a cycle of growth1. It

elicits a strong immune response in man; all tested adult sera from endemic areas have antibodies against this protein1. Our evidence indicates that purified p 190 can alter the course of

parasitaemia in monkeys with falciparum malaria. We have also succeeded in cloning part of the gene for p190 and expressing it in _Escherichia coli_. To this end we have developed a new

technique, antibody select, which greatly simplifies final identification of expressing clones. Access through your institution Buy or subscribe This is a preview of subscription content,

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FALCIPARUM_-INFECTED ERYTHROCYTES TO ABO ANTIGENS DOES NOT AFFECT PFEMP1 AND RIFIN EXPRESSION Article Open access 30 July 2020 GENETIC DISRUPTION OF _PLASMODIUM FALCIPARUM_ MEROZOITE SURFACE

ANTIGEN 180 (PFMSA180) SUGGESTS AN ESSENTIAL ROLE DURING PARASITE EGRESS FROM ERYTHROCYTES Article Open access 28 September 2021 SELECTIVE EXPRESSION OF VARIANT SURFACE ANTIGENS ENABLES

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of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK Roger Hall, John E. Hyde, Mike Goman, David L. Simmons, Ian A. Hope, Martin

Mackay & John Scaife * Pharmaceutical Research Division, Hoffmann La Roche and Co., Basel, Switzerland Bernhard Merkli, Rolf Richle & John Stocker Authors * Roger Hall View author

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A. Hope View author publications You can also search for this author inPubMed Google Scholar * Martin Mackay View author publications You can also search for this author inPubMed Google

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this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hall, R., Hyde, J., Goman, M. _et al._ Major surface antigen gene of

a human malaria parasite cloned and expressed in bacteria. _Nature_ 311, 379–382 (1984). https://doi.org/10.1038/311379a0 Download citation * Received: 19 March 1984 * Accepted: 26 July 1984

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