Immunoglobulin cμ rna in t lymphoma cells is not translated

ABSTRACT It is widely believed that immunoglobulin genes might encode at least part of the receptor for antigen on the T lymphocyte. Evidence supporting this comes from the effects of

anti-immunoglobulin idiotype antibodies on cellular immune networks1–4 and from the presence of idiotypes on immunologically active factors from T cells5–7. Detailed molecular

characterization of the receptor, however, has been seriously hampered by the lack of a suitable cellular source from which it might be isolated. The recent demonstration by Kemp _et al._8,9

that thymocytes and certain cultured lines of mouse T lymphoma cells contain polyadenylated RNA molecules encoded by the immunoglobulin C_μ_ gene (C_μ_ RNA) prompted us to identify the

corresponding protein molecules in those cells. As the haploid mouse genome contains a single C_μ_ gene10, any polypeptide encoded by this gene should react with at least some of the

antibodies present in rabbit anti-mouse IgM antiserum. In this letter we report that a number of T lymphoma lines, regardless of whether they contain C_μ_ RNA, synthesize no detectable _μ_

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IN CD8+ T CELLS PROMOTES ANTITUMOR IMMUNITY Article Open access 20 April 2022 REFERENCES * Eichmann, K. _Eur. J. Immun._ 4, 296–302 (1974). Article  CAS  Google Scholar  * Cosenza, H.,

Julius, M. & Augustin, A. _Immun. Rev._ 34, 3–33 (1977). Article  CAS  Google Scholar  * Eichmann, K. & Rajewsky, K. _Eur. J. Immun._ 5, 661–666 (1975). Article  CAS  Google Scholar

  * Nisonoff, A., Ju, S. T. & Owen, F. _Immun. Rev._ 34, 89–118 (1977). Article  CAS  Google Scholar  * Bach, B. A., Sherman, L., Benacerraf, B. & Greene, M. I. _J. Immun._ 121,

1460–1468 (1978). CAS  PubMed  Google Scholar  * Hirai, Y. & Nisonoff, A. _J. exp. Med._ 151, 1213–1231 (1980). Article  CAS  Google Scholar  * Sy, M. S., Dietz, M. H., Germain, R. N.,

Benacerraf, B. & Greene, M. I. _J. exp. Med._ 151, 1183–1195 (1980). Article  CAS  Google Scholar  * Kemp, D. J., Harris, A. W., Cory, S. & Adams, J. M. _Proc. natn. Acad. Sci.

U.S.A._ 77, 2876–2880 (1980). Article  ADS  CAS  Google Scholar  * Kemp, D. J., Wilson, A., Harris, A. W. & Shortman, K. _Nature_ 286, 168–170 (1980). Article  ADS  CAS  Google Scholar 

* Cory, S. & Adams, J. M. _Cell_ 19, 37–51 (1980). Article  CAS  Google Scholar  * Siden, E. J., Baltimore, D., Clark, D. & Rosenberg, N. E. _Cell_ 16, 389–396 (1979). Article  CAS 

Google Scholar  * Mattes, M. J. & Steiner, L. A. _Nature_ 273, 761–763 (1978). Article  ADS  CAS  Google Scholar  * Ehrlich, H. A., Levinson, J. R., Cohen, S. N. & McDevitt, H. O.

_J. biol. Chem._ 10, 12240–12247 (1979). Google Scholar  * Alwine, J., Kemp, D. J. & Stark, G. R. _Proc. natn. Acad. Sci. U.S.A._ 74, 5350–5354 (1977). Article  ADS  CAS  Google Scholar

  * Kemp, D. J., Harris, A. W. & Adams, J. M. _Proc. natn. Acad. Sci. U.S.A._ (in the press). * klinman, N. R. _et al._ _Annls Immun. Inst. Pasteur, Paris_ 127, 489–495 (1976). CAS 

Google Scholar  * Laemmli, U. K. _Nature_ 227, 680–685 (1970). Article  ADS  CAS  Google Scholar  * Laskey, R. A. & Mills, A. D. _Eur. J. Biochem._ 56, 335–341 (1975). Article  CAS 

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3050, Australia Ian D. Walker & Alan W. Harris Authors * Ian D. Walker View author publications You can also search for this author inPubMed Google Scholar * Alan W. Harris View author

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Immunoglobulin Cμ RNA in T lymphoma cells is not translated. _Nature_ 288, 290–293 (1980). https://doi.org/10.1038/288290a0 Download citation * Received: 12 August 1980 * Accepted: 01

October 1980 * Issue Date: 20 November 1980 * DOI: https://doi.org/10.1038/288290a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get

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