Fusion of human erythrocyte ghosts promoted by the combined action of calcium and phosphate ions

ABSTRACT CALCIUM ions are important in almost all membrane fusion systems1–2. Murayama and Okada3 showed that Sendai virus-induced fusion of Ehrlich ascites cells required Ca2+. When Ehrlich

ascites cells were treated with Sendai virus in the presence of EDTA, cells agglutinated and lysed but did not fuse3,4. Similarly, Ca2+ was required for the fusion of erythrocytes

stimulated by chemicals such as lysolecithin5 or glyceryl monooleate6. Ca2+ plus the ionosphere A23187 (ref. 7) or Ca2+ at high _p_H (ref. 8) promoted the fusion of chicken erythrocytes.

Fusion of phosphatidylserine-rich liposomes was absolutely dependent on the presence of Ca2+9. We describe here the fusion of human erythrocyte ghosts promoted by the combined action of Ca2+

and phosphate buffer. Human erythrocyte ghosts, because they lack cytoplasm and are easily filled with small or large molecules10,11 are useful for the investigation of membrane fusion.

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support SIMILAR CONTENT BEING VIEWED BY OTHERS A SUPRAMOLECULAR SYSTEM MIMICKING THE INFECTION PROCESS OF AN ENVELOPED VIRUS THROUGH MEMBRANE FUSION Article Open access 15 November 2023

STRUCTURAL TRANSITION OF GP64 TRIGGERED BY A PH-SENSITIVE MULTI-HISTIDINE SWITCH Article Open access 03 September 2024 ARCHITECTURE OF THE HUMAN ERYTHROCYTE ANKYRIN-1 COMPLEX Article 14 July

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Jerusalem, Jerusalem, Israel NEHAMA ZAKAI, RICHARD G. KULKA & ABRAHAM LOYTER Authors * NEHAMA ZAKAI View author publications You can also search for this author inPubMed Google Scholar *

RICHARD G. KULKA View author publications You can also search for this author inPubMed Google Scholar * ABRAHAM LOYTER View author publications You can also search for this author inPubMed 

Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE ZAKAI, N., KULKA, R. & LOYTER, A. Fusion of human erythrocyte ghosts promoted by the

combined action of calcium and phosphate ions. _Nature_ 263, 696–699 (1976). https://doi.org/10.1038/263696a0 Download citation * Received: 17 June 1976 * Accepted: 26 July 1976 * Published:

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