Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoitec cell cultures

ABSTRACT CULTURE media for mammalian cells usually require supplementation with serum to supply as yet undefined needs. Because the requirements are likely to be multiple, it is difficult to

distinguish biologically between the effects of nonspecific ‘nutritional’ factors in serum and those of specific regulatory factors. Partial replacement of serum in these systems by

chemically defined substances supplying the nonspecific needs would therefore represent a significant advance towards the definition of such specific factors. We have examined the role of

several serum components in cultures of freshly explanted haemopoietic cells with this goal in mind. Access through your institution Buy or subscribe This is a preview of subscription

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J. GUILBERT & N. N. ISCOVE Authors * L. J. GUILBERT View author publications You can also search for this author inPubMed Google Scholar * N. N. ISCOVE View author publications You can

also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE GUILBERT, L., ISCOVE, N. Partial replacement of serum

by selenite, transferrin, albumin and lecithin in haemopoitec cell cultures. _Nature_ 263, 594–595 (1976). https://doi.org/10.1038/263594a0 Download citation * Received: 22 July 1976 *

Accepted: 27 August 1976 * Issue Date: 14 October 1976 * DOI: https://doi.org/10.1038/263594a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content:

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