Relationship between tritiated poly-l-lysine binding and template activity of human chromosomes

ABSTRACT IT has been reported that cytological preparations of chromosomes can serve as templates in RNA polymerase reactions1–2. The newly synthesised RNA on chromosomes was readily

digested with RNase in contrast to RNA which was synthesised in a similar manner but using as a template denatured DNA immobilised on nitrocellulose filters1. When cytological preparations

of chromosomes were pretreated with poly-L-lysine (PL), no synthesis of RNA could be demonstrated2. The implication was that bound PL blocked those sites on DNA template which were

responsible for transcription. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your

institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access

to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our

FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS GUIDING FUNCTIONS OF THE C-TERMINAL DOMAIN OF TOPOISOMERASE IIΑ ADVANCE MITOTIC CHROMOSOME ASSEMBLY Article Open

access 18 May 2021 NUCLEOSOME DYNAMICS RENDER HETEROCHROMATIN ACCESSIBLE IN LIVING HUMAN CELLS Article Open access 16 May 2025 A CDK-REGULATED CHROMATIN SEGREGASE PROMOTING CHROMOSOME

REPLICATION Article Open access 01 September 2021 REFERENCES * Sederoff, R., Clynes, R., Poncz, M., and Hachtel, S., _J. Cell Biol._, 57, 538 (1973). Article  CAS  Google Scholar  * Umiel,

N., and Plaut, W., _J. Cell Biol._, 56, 139 (1973). Article  CAS  Google Scholar  * Moorhead, P. S., Nowell, P. C., Mellman, W. J., Battips, D. M., and Hungerford, D. A., _Exp Cell Res._,

20, 613 (1960). Article  CAS  Google Scholar  * Evans, H. J., _Br. med. Bull._, 29, 196 (1973). Article  CAS  Google Scholar  * Caspersson, T., Zech, L., Johansson, C., and Modest, E. J.,

_Chromosoma_, 30, 215 (1970). Article  CAS  Google Scholar  * Du Praw, E. J., _DNA and Chromosomes_, 135 (Holt, Rinehart and Winston, New York, 1970). * Hsu, T. C., _Mammal. Chromosome

Newsl._, 13, 43 (1972). Google Scholar  * Seabright, M., _Lancet_, ii, 971 (1971). Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biological Sciences, State

University of New York at Albany, Albany, New York, 12222 S. P. MARFEY & M. G. LI Authors * S. P. MARFEY View author publications You can also search for this author inPubMed Google

Scholar * M. G. LI View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE

MARFEY, S., LI, M. Relationship between tritiated poly-L-lysine binding and template activity of human chromosomes. _Nature_ 249, 559–561 (1974). https://doi.org/10.1038/249559a0 Download

citation * Received: 19 December 1973 * Issue Date: 07 June 1974 * DOI: https://doi.org/10.1038/249559a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative