Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia

ABSTRACT _MLLT10_ (previously called _AF10_) is a moderately common _MLL_ fusion partner predominantly occurring in acute monoblastic leukemia (AML-M5). 10;11 rearrangements require at least

three breaks in order to generate an in-frame _MLL-MLLT10_ fusion as a result of the opposite orientations of both genes on the respective chromosome arms. In this study, we describe a

detailed molecular cytogenetic analysis of _MLL-MLLT10_ positive 10;11 rearrangements in two patients. We observed an as yet unreported chromosomal mechanism with at least four breakpoints,

leading to _MLL-MLLT10_ gene fusion in a 24-year-old male. An inversion of 11q13-q23 with a breakpoint in the _MLL_ gene was followed by an additional break 3′ of _MLL_ prior to insertion of

the 11q segment into _MLLT10_. In a second patient, a 37-year-old male with AML-M5b, molecular cytogenetic analysis of an apparent 10;11 reciprocal translocation showed an intrachromosomal

inversion of 3′_MLLT10_followed by a reciprocal translocation between 10p12 and 11q23. Review of the literature showed that all cases were the result of an inversion of either 10p or 11q

followed by translocation 10p;11q or insertion of the inverted segment into _MLLT10_ or _MLL_. Access through your institution Buy or subscribe This is a preview of subscription content,

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PubMed  Google Scholar  Download references ACKNOWLEDGEMENTS FWO grant Nos G.0028.00 and G.0310.01 and BOF grant No. 011D7699. Heidi Van Limbergen is the recipient of a research grant of the

University of Ghent. Bruce Poppe is supported by a grant of the Fund for Scientific Research, Flanders, Belgium (FWO-Vlaanderen). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Center for

Medical Genetics, Ghent University Hospital, Ghent, Belgium H Van Limbergen, B Poppe, A De Paepe & F Speleman * Department of Hematology, Ghent University Hospital, Ghent, Belgium A

Janssens & L Noens * Department of Hematology, AZ Middelheim, Antwerp, Belgium R De Bock Authors * H Van Limbergen View author publications You can also search for this author inPubMed 

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cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia. _Leukemia_ 16, 344–351 (2002). https://doi.org/10.1038/sj.leu.2402397 Download citation * Received: 13 July 2001 *

Accepted: 22 November 2001 * Published: 04 March 2002 * Issue Date: 01 March 2002 * DOI: https://doi.org/10.1038/sj.leu.2402397 SHARE THIS ARTICLE Anyone you share the following link with

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content-sharing initiative KEYWORDS * _MLL_ * _MLLT10_ * _AF10_ * acute myeloid leukemia * M-FISH