Comparative genomic hybridization as part of a new diagnostic strategy in childhood hyperdiploid acute lymphoblastic leukemia

ABSTRACT The detailed definition of karyotype changes associated with hyperdiploid acute lymphoblastic leukemia (ALL) is a precondition for their exploitation in minimal residual disease

studies with fluorescence _in situ_ hybridization analysis (FISH). In addition, certain karyotype patterns may have different prognostic implications. We have therefore used comparative

genomic hybridization (CGH) to analyze the quantitative karyotype abnormalities in 14 cases of hyperdiploid ALL and correlated the results with those obtained by flow cytometry and

conventional cytogenetic analyses. Despite an overall good agreement between the karyotypes obtained by classical banding techniques and CGH, we came across at least one karyotype

discrepancy per case. Clarification of the discordant findings with fluorescence _in situ_ hybridization (FISH) showed that all stem lines had been correctly defined by CGH. In eight cases,

however, cytogenetic analyses revealed structural abnormalities that were undetectable by CGH. The other discrepancies were mainly due to a cytogenetic misinterpretation of similar sized and

shaped chromosomes. Based on these findings we present a new diagnostic strategy for childhood ALL that includes flow cytometry and classical cytogenetics as well as CGH for the analysis of

aneuploid cases and FISH to resolve the unavoidable discrepancies. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution

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AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Children’s Cancer Research Institute (CCRI), St Anna Children’s Hospital, Vienna, Austria OA Haas & T Henn * Institute of Human Genetics,

University of Kaiserslautern, Kaiserslautern, Germany K Romanakis * National Institutes of Health, Gene Technology Branch, National Center for Human Genome Research, Bethesda, MD, USA S du

Manoir * Molecular Genetics Laboratory, Johns Hopkins Oncology Center, Baltimore, MD, USA C Lengauer Authors * OA Haas View author publications You can also search for this author inPubMed 

Google Scholar * T Henn View author publications You can also search for this author inPubMed Google Scholar * K Romanakis View author publications You can also search for this author

inPubMed Google Scholar * S du Manoir View author publications You can also search for this author inPubMed Google Scholar * C Lengauer View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Haas, O., Henn, T., Romanakis, K. _et al._ Comparative genomic

hybridization as part of a new diagnostic strategy in childhood hyperdiploid acute lymphoblastic leukemia. _Leukemia_ 12, 474–481 (1998). https://doi.org/10.1038/sj.leu.2400943 Download

citation * Received: 30 September 1997 * Accepted: 20 November 1997 * Published: 08 May 1998 * Issue Date: 01 April 1998 * DOI: https://doi.org/10.1038/sj.leu.2400943 SHARE THIS ARTICLE

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by the Springer Nature SharedIt content-sharing initiative KEYWORDS * hyperdiploid ALL * flow cytometry * cytogenetics * fluorescence _in situ_ hybridization (FISH) * comparative genomic

hybridization (CGH)