In vitro cytocidal effect of l-glutaminase on leukaemic lymphocytes

ABSTRACT MOUSE lymphomas, such as 6C3HED, undergo complete regression after one or more injections of L-asparaginase1, and remissions have been reported in some patients with acute leukaemia

after treatment with L-asparaginase2–4. Roberts _et al._5 found that purified bacterial L-glutaminase inhibited the growth of Ehrlich mouse carcinoma. Before considering this enzyme for

therapeutic studies in man, it is necessary to study _in vitro_ effects of the enzyme on human blood cells. Previous work6 demonstrated that the cells of 6C3HED lymphoma do not survive

incubation with very low concentrations of L-asparaginase (1.7 mIU/ml.), while the cells of the _in vivo_ resistant variant of 6C3HED were not killed by 170 mIU of enzyme/ml. The blood

lymphocytes from patients with chronic lymphocytic leukaemia were found to be more sensitive than normal lymphocytes to incubation with 170 mIU of L-asparaginase/ml. but not as sensitive as

6C3HED mouse lymphoma which responds _in vivo_ to the enzyme. In this study, the slide chamber method7 was used to compare the sensitivity of normal and leukaemic lymphocytes to glutaminase.

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support SIMILAR CONTENT BEING VIEWED BY OTHERS DIRECT ADMINISTRATION OF HUMAN LEUCOCYTE ANTIGEN (DHLA) MOLECULES INTO TUMOUR SITES: PROPOSAL FOR A NEW IMMUNOTHERAPY FOR CANCER Article Open

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Administration Hospital, Hines, Illinois, 60141 ROBERT SCHREK * Departments of Experimental Animal Medicine and Medicine, University of Washington School of Medicine, Seattle, Washington,

98105 JOHN S. HOLCENBERG, JOSEPH ROBERTS & WILLIAM C. DOLOWY Authors * ROBERT SCHREK View author publications You can also search for this author inPubMed Google Scholar * JOHN S.

HOLCENBERG View author publications You can also search for this author inPubMed Google Scholar * JOSEPH ROBERTS View author publications You can also search for this author inPubMed Google

Scholar * WILLIAM C. DOLOWY View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE SCHREK, R., HOLCENBERG, J., ROBERTS, J. _et al._ _In vitro_ Cytocidal Effect of L-Glutaminase on Leukaemic Lymphocytes. _Nature_ 232, 265 (1971). https://doi.org/10.1038/232265a0

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