Ribosomal binding of aug by formyl-methionyl-srna

ABSTRACT IT has been shown that a triplet can facilitate the binding of an amino acyl-_s_RNA molecule to a ribosome1, and, conversely, that an _s_RNA molecule can bind a labelled triplet to

a ribosome2. Recently, Kondo _et al._3 have shown that, in the presence of the necessary components, formyl-methionyl-_s_RNAF is required to bind the f2 phage RNA to 70_S_ ribosomes at 5 mM

Mg++. In this communication we describe how labelled pApUpG can be bound in a stable complex to ribosomes in the presence of formyl-methionyl-_s_RNAF, there being one molecule of triplet

bound per molecule of _s_RNA bound. When puromycin is added, the formyl-methionyl residue is rapidly removed and the labelled triplet is more slowly lost from the ribosome. Access through

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ADS  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * MRC Laboratory of Molecular Biology, Hills Road, Cambridge M. S. BRETSCHER Authors * M. S.

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M. Ribosomal Binding of AUG by Formyl-methionyl-_s_RNA. _Nature_ 220, 1233–1234 (1968). https://doi.org/10.1038/2201233a0 Download citation * Received: 06 November 1968 * Issue Date: 21

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