Freeze-drying of bovine spermatozoa

ABSTRACT SINCE 1956, increased attention has been given to techniques for freeze-drying bovine spermatozoa as a method for long-term semen preservation1–5. In one approach1–3, spermatozoa

were dried in vacuum after being diluted, glyeerolated, equilibrated and slowly frozen. Low survival-rates and frequent failures were encountered. Leidl3 suggested three possible causes:

(_a_) concentration of glycerol to toxic levels; (_b_) excessive removal of water; (_c_) morphological damage (occasional broken mid-pieces). Access through your institution Buy or subscribe

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METHOD FOR LONG-TERM ROOM TEMPERATURE STORAGE OF MOUSE FREEZE-DRIED SPERM Article Open access 02 January 2025 WHOLE GENOME INTEGRITY AND ENHANCED DEVELOPMENTAL POTENTIAL IN RAM FREEZE-DRIED

SPERMATOZOA AT MILD SUB-ZERO TEMPERATURE Article Open access 02 November 2020 DRYING AND TEMPERATURE INDUCED CONFORMATIONAL CHANGES OF NUCLEIC ACIDS AND STALLION SPERM CHROMATIN IN TREHALOSE

PRESERVATION FORMULATIONS Article Open access 07 July 2021 REFERENCES * Albright, J. L., Erb, R. E., and Ehlers, M. H., _J. Dairy Sci._, 41, 206 (1958). Article  Google Scholar  * Bialy,

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INFORMATION AUTHORS AND AFFILIATIONS * Dairy Breeding Research Center, Department of Dairy Science, Pennsylvania State University, University Park, R. G. SAACKE & J. O. ALMQUIST Authors

* R. G. SAACKE View author publications You can also search for this author inPubMed Google Scholar * J. O. ALMQUIST View author publications You can also search for this author inPubMed 

Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE SAACKE, R., ALMQUIST, J. Freeze-drying of Bovine Spermatozoa. _Nature_ 192, 995–996

(1961). https://doi.org/10.1038/192995a0 Download citation * Issue Date: 09 December 1961 * DOI: https://doi.org/10.1038/192995a0 SHARE THIS ARTICLE Anyone you share the following link with

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