Purification and concentration of dye-protein conjugates by gel filtration

ABSTRACT IT was shown by Porath and Flodin1 that filtration through cross-linked dextran gels served as a simple method for the separation of materials of varying molecular size. This

procedure has now been found to be of particular value for the purification of antibody-fluorescent dye conjugates for use in the Coon's2 technique, since unconjugated dye and dye

impurities are removed so that non-specific staining of the tissues has thereby been greatly reduced or abolished. Access through your institution Buy or subscribe This is a preview of

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SOLUTIONS IN PROTEIN A PURIFICATION IMPROVES PHYSICOCHEMICAL PROPERTIES OF MONOCLONAL ANTIBODIES Article Open access 19 January 2021 SPECIFIC, SENSITIVE AND QUANTITATIVE PROTEIN DETECTION BY

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INFORMATION AUTHORS AND AFFILIATIONS * Department of Experimental Pathology, University of Birmingham, W. GEORGE & K. W. WALTON Authors * W. GEORGE View author publications You can also

search for this author inPubMed Google Scholar * K. W. WALTON View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE GEORGE, W., WALTON, K. Purification and Concentration of Dye-Protein Conjugates by Gel Filtration. _Nature_ 192, 1188–1189 (1961).

https://doi.org/10.1038/1921188a0 Download citation * Issue Date: 23 December 1961 * DOI: https://doi.org/10.1038/1921188a0 SHARE THIS ARTICLE Anyone you share the following link with will

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