Presenilin is required for activity and nuclear access of notch in drosophila

ABSTRACT Presenilins are membrane proteins with multiple transmembrane domains that are thought to contribute to the development of Alzheimer's disease by affecting the processing of

β-amyloid precursor protein1. Presenilins also facilitate the activity of transmembrane receptors of the LIN-12/Notch family2,3,4,5. After ligand-induced processing, the intracellular domain

of LIN-12/Notch can enter the nucleus and participate in the transcriptional control of downstream target genes6,7,8,9. Here we show that null mutations in the _Drosophila Presenilin_ gene

abolish Notch signal transduction and prevent its intracellular domain from entering the nucleus. Furthermore, we provide evidence that presenilin is required for the proteolytic release of

the intracellular domain from the membrane following activation of Notch by ligand. Access through your institution Buy or subscribe This is a preview of subscription content, access via

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characterization of the PS alleles as neurogenic mutations; A. Adachi, R.Perez and X.-J. Qin for technical assistance; S. T. Crews, T. Lieber and P. M. Macdonald for antisera; and R. Axel,

D. Brower, S.-K. Chan, H.-M. Chung and M. Zecca for advice and discussion. G.S. is an investigator and I.G. is an associate investigator of the Howard Hughes Medical Institute. AUTHOR

INFORMATION AUTHORS AND AFFILIATIONS * Departments of Genetics and Development, New York, 10032, New York, USA Gary Struhl * Departments of Howard Hughes Medical Institute, Columbia

University, College of Physicians and Surgeons, New York, 10032, New York, USA Gary Struhl & Iva Greenwald * Departments of Biochemistry and Molecular Biophysics, New York, 10032, New

York, USA Iva Greenwald Authors * Gary Struhl View author publications You can also search for this author inPubMed Google Scholar * Iva Greenwald View author publications You can also

search for this author inPubMed Google Scholar CORRESPONDING AUTHORS Correspondence to Gary Struhl or Iva Greenwald. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE

THIS ARTICLE Struhl, G., Greenwald, I. Presenilin is required for activity and nuclear access of Notch in _Drosophila_. _Nature_ 398, 522–525 (1999). https://doi.org/10.1038/19091 Download

citation * Received: 22 February 1999 * Accepted: 15 March 1999 * Issue Date: 08 April 1999 * DOI: https://doi.org/10.1038/19091 SHARE THIS ARTICLE Anyone you share the following link with

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