Comparison of medulloblastoma and normal neural transcriptomes identifies a restricted set of activated genes

ABSTRACT Over 1.4 million transcript tags expressed in 20 different human medulloblastomas were counted using serial analysis of gene expression. Digital gene expression profiles in the

medulloblastoma were compared to multiple regions of the normal human brain, revealing 30 transcripts with high expression in multiple tumors and little or no expression in the normal

cerebellum and other adult and pediatric brain regions. Using independent medulloblastoma samples and normal tissue, real-time PCR verified eight of nine selected genes as candidate

tumor-associated antigens. Differential protein expression for CD24, prolactin and Topo2A was further confirmed by immunohistochemical analysis using medulloblastoma and normal brain

sections and a tissue microarray. The genes highly expressed in the medulloblastoma include PRAME, a cancer-testis antigen and potential targets for immunotherapy. Access through your

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_Science_, 270, 484–487. Download references ACKNOWLEDGEMENTS Medulloblastoma samples were provided by the Duke Brain Tumor Bank and the Pediatric Oncology Group via the NIH Collaborative

Human Tissue Network. We thank Jennifer Shoemaker for statistics consulting, Dr Roger McLendon (Duke University Medical Center) for expert histopathology, Drs Anita Lal, Robert Beaty, Brian

Fee, Keith Killian and Janete Cerutti for providing SAGE data on normal tissues, Dennis W Rickman for expert immunohistochemistry assistance, and Dr Steven Jones, Jacquie Schein and members

of the BCGSC sequencing team for expert technical assistance. Funding was provided by the Cancer Genome Anatomy Project (NCI contract S98-146), NCI Grant U01 CA88128 and the Pediatric Brain

Tumor Foundation of the United States. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pathology, Duke University Medical Center, Box 3156, Durham, 27710, NC, USA Kathy Boon, 

Jennifer B Edwards, I-Mei Siu, Deric Olschner & Gregory J Riggins * Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, 21205, MD, USA Charles G Eberhart *

Sequencing Center, BC Cancer Agency, Vancouver, Canada Marco A Marra * Office of Cancer Genomics, National Cancer Institute, Bethesda, 20892, MD, USA Robert L Strausberg Authors * Kathy Boon

View author publications You can also search for this author inPubMed Google Scholar * Jennifer B Edwards View author publications You can also search for this author inPubMed Google

Scholar * I-Mei Siu View author publications You can also search for this author inPubMed Google Scholar * Deric Olschner View author publications You can also search for this author

inPubMed Google Scholar * Charles G Eberhart View author publications You can also search for this author inPubMed Google Scholar * Marco A Marra View author publications You can also search

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ARTICLE CITE THIS ARTICLE Boon, K., Edwards, J., Siu, IM. _et al._ Comparison of medulloblastoma and normal neural transcriptomes identifies a restricted set of activated genes. _Oncogene_

22, 7687–7694 (2003). https://doi.org/10.1038/sj.onc.1207043 Download citation * Received: 24 June 2003 * Revised: 25 July 2003 * Accepted: 25 July 2003 * Published: 23 October 2003 * Issue

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tumor-associated antigens * SAGE * bioinformatics