Induced genomic instability in irradiated germ cells and in the offspring; reconciling discrepancies among the human and animal studies

ABSTRACT Many studies confirmed that radiation induces genomic instability in whole-body systems. However, the results of the studies are not always consistent with each other. Attempts are

made in the present review to resolve the discrepancies. Many of the studies in human and experimental animals utilize the length change mutation of minisatellite sequences as a marker of

genomic instability. Minisatellite sequences frequently change their length, and the data obtained by conventional Southern blotting give rather qualitative information, which is sometimes

difficult to scrutinize quantitatively. This is the problem inevitably associated with the study of minisatellite mutations and the source of some conflicts among studies in humans and mice.

Radiation induction of genomic instability has also been assessed in whole-body experimental systems, using other markers such as the mouse pink-eyed unstable allele and the specific

pigmentation loci of medaka fish (_Oryzias latipes_). Even though there are some contradictions, all these studies have demonstrated that genomic instability is induced in the germ cells of

irradiated parents, especially of males, and in offspring born to them. Among these, transmission of genomic instability to the second generation of irradiated parents is limited to the

mouse minisatellite system, and awaits further clarification in other experimental systems. Access through your institution Buy or subscribe This is a preview of subscription content, access

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Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS RATS EXHIBIT AGE-RELATED MOSAIC LOSS OF CHROMOSOME Y

Article Open access 21 December 2021 DISSECTING SINGLE-CELL GENOMES THROUGH THE CLONAL ORGANOID TECHNIQUE Article Open access 18 October 2021 GENOMIC STABILITY OF MOUSE SPERMATOGONIAL STEM

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Mol. Mutagen._, 36, 134–145. Download references ACKNOWLEDGEMENTS This work is supported by a Grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan,

and by a grant from the Nuclear Safety Research Association. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Radiation Biology Center, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto,

606-8501, Japan Ohtsura Niwa Authors * Ohtsura Niwa View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Ohtsura Niwa.

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minisatellite * transgenerational