Increased transversions in a novel mutator colon cancer cell line

ABSTRACT We describe a novel mutator phenotype in the Vaco411 colon cancer cell line which increases the spontaneous mutation rate 10–100-fold over background. This mutator results primarily

in transversion base substitutions which are found infrequently in repair competent cells. Of the four possible types of transversions, only three were principally recovered. Spontaneous

mutations recovered also included transitions and large deletions, but very few frameshifts were recovered. When compared to known mismatch repair defective colon cancer mutators, the

distribution of mutations in Vaco411 is significantly different. Consistent with this difference, Vaco411 extracts are proficient in assays of mismatch repair. The Vaco411 mutator appears to

be novel, and is not an obvious human homologue of any of the previously characterized bacterial or yeast transversion phenotypes. Several hypotheses by which this mutator may produce

transversions are presented. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your

institution Subscribe to this journal Receive 50 print issues and online access $259.00 per year only $5.18 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access

to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our

FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS INHERITED _MUTYH_ MUTATIONS CAUSE ELEVATED SOMATIC MUTATION RATES AND DISTINCTIVE MUTATIONAL SIGNATURES IN NORMAL

HUMAN CELLS Article Open access 08 July 2022 RATE VOLATILITY AND ASYMMETRIC SEGREGATION DIVERSIFY MUTATION BURDEN IN CELLS WITH MUTATOR ALLELES Article Open access 04 January 2021 PERVASIVE

LESION SEGREGATION SHAPES CANCER GENOME EVOLUTION Article 24 June 2020 AUTHOR INFORMATION Author notes * James R Eshleman Present address: Department of Pathology, Ross 632, AUTHORS AND

AFFILIATIONS * Department of Pathology, University Hospitals of Cleveland and Case Western Reserve University, Suite 200, UCRC II, 11001 Cedar Road, Cleveland, 44106, Ohio, USA James R

Eshleman * Department of Medicine, University Hospitals of Cleveland and Case Western Reserve University, Suite 200, UCRC II, 11001 Cedar Road, Cleveland, 44106, Ohio, USA P Scott Donover, 

Sandra E Swinler, James D Lutterbaugh, James KV Willson, W David Sedwick & Sanford D Markowitz * Department of General Medical Sciences and Ireland Cancer Center, University Hospitals of

Cleveland and Case Western Reserve University, Suite 200, UCRC II, 11001 Cedar Road, Cleveland, 44106, Ohio, USA Martina L Veigl * Department of Biochemistry and Howard Hughes Medical

Institute, Nanaline Duke Building, Durham, 27710, North Carolina, USA Guo-Min Li & Paul Modrich * Department of Medicine-Oncology, Duke University Medical Center, Nanaline Duke Building,

Durham, 27710, North Carolina, USA Susan J Littman * Johns Hopkins Medical Institutions, 720 Rutland Avenue, Baltimore, 21205, Maryland, USA James R Eshleman * Department of Pathology,

University of Kentucky Medical Center, Lexington, 40536, Kentucky, USA Guo-Min Li Authors * James R Eshleman View author publications You can also search for this author inPubMed Google

Scholar * P Scott Donover View author publications You can also search for this author inPubMed Google Scholar * Susan J Littman View author publications You can also search for this author

inPubMed Google Scholar * Sandra E Swinler View author publications You can also search for this author inPubMed Google Scholar * Guo-Min Li View author publications You can also search for

this author inPubMed Google Scholar * James D Lutterbaugh View author publications You can also search for this author inPubMed Google Scholar * James KV Willson View author publications You

can also search for this author inPubMed Google Scholar * Paul Modrich View author publications You can also search for this author inPubMed Google Scholar * W David Sedwick View author

publications You can also search for this author inPubMed Google Scholar * Sanford D Markowitz View author publications You can also search for this author inPubMed Google Scholar * Martina

L Veigl View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Eshleman,

J., Donover, P., Littman, S. _et al._ Increased transversions in a novel mutator colon cancer cell line. _Oncogene_ 16, 1125–1130 (1998). https://doi.org/10.1038/sj.onc.1201629 Download

citation * Received: 18 June 1997 * Revised: 07 October 1997 * Accepted: 07 October 1997 * Published: 10 March 1998 * Issue Date: 05 March 1998 * DOI: https://doi.org/10.1038/sj.onc.1201629

SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to

clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * colon cancer * HPRT * mutator phenotype * transversions