Regulation of the specific dna binding activity of xenopus laevis p53: evidence for conserved regulation through the carboxy-terminus of the protein

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Regulation of the specific dna binding activity of xenopus laevis p53: evidence for conserved regulation through the carboxy-terminus of the protein"


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ABSTRACT Recombinant human p53 isolated either from _E. COLI_ or from insect cells is poorly active for binding to DNA but it can be dramatically stimulated by phosphorylation, antibody


binding to the carboxy-terminal negative regulatory domain, short peptides derived from this negative regulatory domain or short single strands of DNA. We report here that _XENOPUS_ p53 has


a very similar behavior. Using a new set of monoclonal antibodies directed either to the amino- or the carboxy-terminus of _XENOPUS_ p53, we demonstrate that the frog protein can be


activated by specific carboxy-terminus monoclonal antibodies in order to bind to human p53 DNA response element. In addition, we report that such activation of both humans and frogs protein


can also be achieved by small peptides derived from the carboxy-terminus of both p53. Although, the sequence of this region is not conserved in the various p53 species, the presence of


conserved basic residues indicates that such activation is charge-dependent. This is confirmed by the finding that small poly-lysine peptides can activate both human and _XENOPUS_ p53. _IN


VIVO_ expression of _XENOPUS_ p53 indicates that this protein is able to transactivate a wide variety of human p53 response elements as long as the experiments are performed at 32°C since


activity at 37°C, a temperature well above the natural temperature of _XENOPUS_, is lost. Finally, we demonstrate that human mdm2 is able to down regulate the transcriptional activity of


_XENOPUS_ p53. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution


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Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS STRUCTURAL DIVERSITY OF P63 AND P73 ISOFORMS Article Open access 21 March 2022 EVOLUTIONARY HISTORY OF THE P53 FAMILY


DNA-BINDING DOMAIN: INSIGHTS FROM AN _ALVINELLA POMPEJANA_ HOMOLOG Article Open access 07 March 2022 DESIGNED ANKYRIN REPEAT PROTEINS AS A TOOL BOX FOR ANALYZING P63 Article Open access 18


June 2022 AUTHOR INFORMATION Author notes * Anne-Claire Hardy Bessard Present address: Unité d'Oncologie Médicale, Hopital Saint Louis, avenue C. Vellefaux, Paris, 75010, France * Edith


Garay Present address: Centro de Neurobiologia, Apartado Postal 1–1141, 76001, Queretaro, Qro., C.P., Mexique * Yann Legros Present address: Pharmacia Biotech Parc Technologique, rue René


Razel, Saclay, Orsay Cedex, 91898, France * Thierry Soussi Present address: UMR 218 du CNRS, Institut Curie, Pavillon Trouillet Rossignol, 26 rue d'Ulm, Paris, cedex 05 75231, France


AUTHORS AND AFFILIATIONS * U301 INSERM, Institut de Génétique Moléculaire 27, rue J Dodu, Paris, 75010 Anne-Claire Hardy Bessard, Edith Garay, Virginie Lacronique, Yann Legros, Christel


Demarquay, Armelle Houque & Thierry Soussi * CNRS UMR 9921 UFR Pharmacie 15 Av Flahault, Montpellier, Cedex 01 34060, France Jean Michel Portefaix & Claude Granier Authors *


Anne-Claire Hardy Bessard View author publications You can also search for this author inPubMed Google Scholar * Edith Garay View author publications You can also search for this author


inPubMed Google Scholar * Virginie Lacronique View author publications You can also search for this author inPubMed Google Scholar * Yann Legros View author publications You can also search


for this author inPubMed Google Scholar * Christel Demarquay View author publications You can also search for this author inPubMed Google Scholar * Armelle Houque View author publications


You can also search for this author inPubMed Google Scholar * Jean Michel Portefaix View author publications You can also search for this author inPubMed Google Scholar * Claude Granier View


author publications You can also search for this author inPubMed Google Scholar * Thierry Soussi View author publications You can also search for this author inPubMed Google Scholar RIGHTS


AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bessard, AC., Garay, E., Lacronique, V. _et al._ Regulation of the specific DNA binding activity of _XENOPUS


LAEVIS_ p53: evidence for conserved regulation through the carboxy-terminus of the protein. _Oncogene_ 16, 883–890 (1998). https://doi.org/10.1038/sj.onc.1201598 Download citation *


Received: 06 August 1997 * Revised: 26 September 1997 * Accepted: 26 September 1997 * Published: 03 March 1998 * Issue Date: 19 February 1998 * DOI: https://doi.org/10.1038/sj.onc.1201598


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clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * p53 * xenopus * manoclonal antibodies * transactivation


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