Rapid induction of b-cell lymphomas in mice carrying a human igh/c-mycyac

ABSTRACT Activation of the c-_MYC_ proto-oncogene by one of the immunoglobulin (Ig) loci after chromosomal translocation is a consistent feature of Burkitt's lymphoma. Different

subtypes of this tumor vary in the molecular architecture of the translocation region. In most cases there are no known regulatory elements of the Ig locus neighboring the oncogene and this

considerably obscures the mechanism of its deregulation. In order to assess possible oncogene activation signals, we produced an experimental translocation region by insertion of a c-_MYC_

gene about 50 kb from the IgH intron enhancer in a yeast artificial chromosome (YAC) containing a 220 kb region of the human Ig heavy chain (IgH) locus. Single copy integration of this YAC

into the genome of mouse embryonic stem (ES) cells was achieved by spheroplast fusion. Chimeric mice derived from these ES cells developed monoclonal B-cell lymphomas expressing surface IgM

by 8 – 16 weeks of age. The IgH/c-_MYC_ translocus showed different VHDJH rearrangement in almost all tumors without any alterations of the distance between c-_MYC_ and the IgH intron

enhancer. This mouse model can be used for the _IN VIVO_ analysis of c-_MYC_ deregulation and the tumor formation capacity of the IgH locus in aberrant rearrangements. Access through your

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BEING VIEWED BY OTHERS DELETION OF THE TRANSCRIPTIONAL REGULATOR TFAP4 ACCELERATES C-MYC-DRIVEN LYMPHOMAGENESIS Article Open access 09 March 2023 AN ERG-DRIVEN TRANSCRIPTIONAL PROGRAM

CONTROLS B CELL LYMPHOPOIESIS Article Open access 15 June 2020 T-ALL CAN EVOLVE TO ONCOGENE INDEPENDENCE Article 22 January 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of

Development and Genetics, The Babraham Institute, Babraham, Cambridge, CB2 4AT, UK Christoph Bützler, Xiangang Zou, Andrei V Popov & Marianne Brüggemann Authors * Christoph Bützler View

author publications You can also search for this author inPubMed Google Scholar * Xiangang Zou View author publications You can also search for this author inPubMed Google Scholar * Andrei V

Popov View author publications You can also search for this author inPubMed Google Scholar * Marianne Brüggemann View author publications You can also search for this author inPubMed Google

Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bützler, C., Zou, X., Popov, A. _et al._ Rapid induction of B-cell lymphomas in mice carrying a

human IgH/c-_MYC_YAC. _Oncogene_ 14, 1383–1388 (1997). https://doi.org/10.1038/sj.onc.1200968 Download citation * Received: 05 September 1996 * Revised: 18 November 1996 * Accepted: 18

November 1996 * Issue Date: 20 March 1997 * DOI: https://doi.org/10.1038/sj.onc.1200968 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get

shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * c-_myc_

deregulation * chromosomal translocation * Burkitt's lymphoma * translocus control * immunoglobulin intron enhancer