Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats

ABSTRACT OBJECTIVE: To explore the effects of transient correction of enhanced corticoadrenal activity in monosodium L-glutamate (MSG)-damaged female rats on peripheral insulin sensitivity

and _in vitro_ retroperitoneal (RP) adipocyte function. DESIGNS: A dose of 4 mg/g body weight (BW) of MSG or vehicle (CTR) was i.p. injected, once every 2 days, between days 2 and 10 of age,

in female rats. Intact and 21 day-operated (sham or adrenal enucleation (AE)) rats from both (CTR and MSG) groups were used for experimentation on day 120 of age. Circulating levels of

several hormones, in basal and after i.v. high-glucose load conditions, and RP adiposity morphology and function were then evaluated. RESULTS: MSG rats developed increased adrenocortical

function, hyperadiposity, hyperleptinemia, hyperinsulinemia and decreased peripheral insulin sensitivity. These characteristics were fully reversed after transient correction of

corticoadrenal hyperactivity induced by AE. In addition, _in vitro_ experimentation with isolated RP adipocytes indicated that cells from intact MSG animals displayed decreased sensitivity

to insulin and dexamethasone stimulation of leptin secretion. Interestingly, adipocyte dysfunction in MSG rats was fully abrogated after AE-induced transient correction of insulinemia,

leptinemia and adrenocortical activity. Importantly, the reversion of these metabolic abnormalities, induced by AE for 21 days, in MSG animals did occur, despite no significant changes in BW

values. CONCLUSION: Our results support that the changes in adipocyte characteristics and peripheral insulin resistance, developed in this pseudo-obese female rat model, are mainly due to

increased glucocorticoid production. Importantly, appropriate correction of the enhanced adrenocortical activity fully reversed these abnormal functions. Access through your institution Buy

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indebted to Ing. O Vercellini for animal care and Mrs C Ferese for histological preparations. The editorial assistance of Mrs SH Rogers in the correction of the manuscript is deeply

recognized. This study was partly supported by grants from the Fondation de Recherche en Endocrinologie 05/06 (ES), FONCyT (PICT 5191/99) (ES), UNLP (11/M086) (GC) and FNSR (3200BO-105657/1)

(RCG). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Neuroendocrine Unit, Multidisciplinary Institute on Cell Biology (CONICET-CICPBA), La Plata, Argentina G Moreno, M Perelló & E

Spinedi * Department of Histology and Embryology ‘B’, School of Medicine, UNLP-CICPBA, La Plata, Argentina G Camihort, G Luna & G Console * Division of Endocrinology, Diabetology &

Metabolism, University Hospital (CHUV), Lausanne, Switzerland R C Gaillard Authors * G Moreno View author publications You can also search for this author inPubMed Google Scholar * M Perelló

View author publications You can also search for this author inPubMed Google Scholar * G Camihort View author publications You can also search for this author inPubMed Google Scholar * G

Luna View author publications You can also search for this author inPubMed Google Scholar * G Console View author publications You can also search for this author inPubMed Google Scholar * R

C Gaillard View author publications You can also search for this author inPubMed Google Scholar * E Spinedi View author publications You can also search for this author inPubMed Google

Scholar CORRESPONDING AUTHOR Correspondence to E Spinedi. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Moreno, G., Perelló, M., Camihort, G. _et al._

Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats. _Int J Obes_ 30, 73–82 (2006). https://doi.org/10.1038/sj.ijo.0803109

Download citation * Received: 30 November 2004 * Revised: 29 June 2005 * Accepted: 28 July 2005 * Published: 11 October 2005 * Issue Date: 01 January 2006 * DOI:

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currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * MSG * fat morphometry * glucose * ACTH * glucocorticoid

* insulin * leptin