Downregulation of id4 by promoter hypermethylation in gastric adenocarcinoma
Downregulation of id4 by promoter hypermethylation in gastric adenocarcinoma"
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ABSTRACT Promoter hypermethylation has become apparent as a common mechanism of gene silencing in cancer. Based on our published microarray expression data, we noticed a prominent
downregulation of _ID4_ in gastric adenocarcinoma. The dense 5′ CpG island covering the previously mapped upstream promoter of _ID4_ has prompted us to relate its downregulation to promoter
hypermethylation. ID proteins are distinct members in the helix–loop–helix family of transcriptional regulators, which modulate various key developmental processes. Emerging data have
suggested the involvement of _ID_ genes in tumorigenesis. In this study using bisulfite genomic sequencing, we have found hypermethylation of _ID4_ promoter in most gastric cancer cell lines
and 30% of primary tumors. This correlated with decreased level of _ID4_ expression. Restoration of _ID4_ expression in various gastric cancer cell lines was achieved by treatment with the
DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine, which at times required the synergistic action of the histone deacetylase inhibitor trichostatin A, but not with trichostatin A alone.
Re-expression was accompanied by the corresponding _ID4_ promoter demethylation. Furthermore, we have found significant association of _ID4_ promoter methylation with _hMLH1_ promoter
methylation (_P_=0.008) and microsatellite instability (_P_=0.006). Overall, our results have shown that transcriptional silencing of _ID4_ is related to the aberrant methylation of its
promoter in gastric cancer. The significant association of _ID4_ and _hMLH1_ promoter hypermethylation suggested that _ID4_ may also be among the genes being targeted in the CpG island
methylator phenotype tumorigenic pathway. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access
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subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS TRANSCRIPTIONAL OVERLAP LINKS DNA HYPOMETHYLATION WITH DNA HYPERMETHYLATION AT ADJACENT
PROMOTERS IN CANCER Article Open access 30 August 2021 DNMT3B OVEREXPRESSION DOWNREGULATES GENES WITH CPG ISLANDS, COMMON MOTIFS, AND TRANSCRIPTION FACTOR BINDING SITES THAT INTERACT WITH
DNMT3B Article Open access 02 December 2022 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED TUMOUR-RELATED GENES REGULATED BY UHRF1-DRIVEN DNA METHYLATION Article Open access 07 August 2024
REFERENCES * Arnold JM, Mok SC, Purdie D and Chenevix-Trench G . (2001). _Br. J. Cancer_, 84, 352–359. * Bain G, Quong MW, Soloff RS, Hedrick SM and Murre C . (1999). _J. Exp. Med._, 190,
1605–1616. * Beger C, Pierce LN, Kruger M, Marcusson EG, Robbins JM, Welcsh P, Welch PJ, Welte K, King MC, Barber JR and Wong-Staal F . (2001). _Proc. Natl. Acad. Sci. USA_, 98, 130–135. *
Benezra R, Rafii S and Lyden D . (2001). _Oncogene_, 20, 8334–8341. * Bounpheng MA, Dimas JJ, Dodds SG and Christy BA . (1999). _FASEB J._, 13, 2257–2264. * Cameron EE, Bachman KE, Myohanen
S, Herman JG and Baylin SB . (1999). _Nat. Genet._, 21, 103–107. * Cory S and Adams JM . (2002). _Nat. Rev. Cancer_, 2, 647–656. * Deleu S, Savonet V, Behrends J, Dumont JE and Maenhaut C .
(2002). _Exp. Cell Res._, 279, 62–70. * Deng G, Chen A, Hong J, Chae HS and Kim YS . (1999). _Cancer Res._, 59, 2029–2033. * Jen Y, Manova K and Benezra R . (1996). _Dev. Dyn._, 207,
235–252. * Jen Y, Manova K and Benezra R . (1997). _Dev. Dyn._, 208, 92–106. * Ji J, Chen X, Leung SY, Chi JT, Chu KM, Yuen ST, Li R, Chan AS, Li J, Dunphy N and So S . (2002). _Oncogene_,
21, 6549–6556. * Kee Y and Bronner-Fraser M . (2001). _Mech. Dev._, 109, 341–345. * Kleeff J, Ishiwata T, Friess H, Buchler MW, Israel MA and Korc M . (1998). _Cancer Res._, 58, 3769–3772. *
Leung SY, Chen X, Chu KM, Yuen ST, Mathy J, Ji J, Chan AS, Li R, Law S, Troyanskaya OG, Tu IP, Wong J, So S, Botstein D and Brown PO . (2002). _Proc. Natl. Acad. Sci. USA_, 99, 16203–16208.
* Leung SY, Yuen ST, Chung LP, Chu KM, Chan AS and Ho JC . (1999a). _Cancer Res._, 59, 159–164. * Leung SY, Yuen ST, Chung LP, Chu KM, Wong MP, Branicki FJ and Ho JC . (1999b). _Br. J.
Cancer_, 79, 582–588. * Lorincz MC, Schubeler D, Goeke SC, Walters M, Groudine M and Martin DI . (2000). _Mol. Cell. Biol._, 20, 842–850. * Massari ME and Murre C . (2000). _Mol. Cell.
Biol._, 20, 429–440. * Morrow MA, Mayer EW, Perez CA, Adlam M and Siu G . (1999). _Mol. Immunol._, 36, 491–503. * Pagliuca A, Cannada-Bartoli P and Lania L . (1998). _J. Biol. Chem._, 273,
7668–7674. * Paulin R, Grigg GW, Davey MW and Piper AA . (1998). _Nucleic Acids Res._, 26, 5009–5010. * Rivera R and Murre C . (2001). _Oncogene_, 20, 8308–8316. * Toyota M, Ahuja N,
Ohe-Toyota M, Herman JG, Baylin SB and Issa JP . (1999a). _Proc. Natl. Acad. Sci. USA_, 96, 8681–8686. * Toyota M, Ahuja N, Suzuki H, Itoh F, Ohe-Toyota M, Imai K, Baylin SB and Issa JP .
(1999b). _Cancer Res._, 59, 5438–5442. * Turker MS . (2002). _Oncogene_, 21, 5388–5393. * Vandeputte DA, Troost D, Leenstra S, Ijlst-Keizers H, Ramkema M, Bosch DA, Baas F, Das NK and
Aronica E . (2002). _Glia_, 38, 329–338. * Veigl ML, Kasturi L, Olechnowicz J, Ma AH, Lutterbaugh JD, Periyasamy S, Li GM, Drummond J, Modrich PL, Sedwick WD and Markowitz SD . (1998).
_Proc. Natl. Acad. Sci. USA_, 95, 8698–8702. * Welcsh PL, Lee MK, Gonzalez-Hernandez RM, Black DJ, Mahadevappa M, Swisher EM, Warrington JA and King MC . (2002). _Proc. Natl. Acad. Sci.
USA_, 99, 7560–7565. * Wice BM and Gordon JI . (1998). _J. Biol. Chem._, 273, 25310–25319. * Wilson JW, Deed RW, Inoue T, Balzi M, Becciolini A, Faraoni P, Potten CS and Norton JD . (2001).
_Cancer Res._, 61, 8803–8810. * Yuen ST, Chan TL, Ho JW, Chan AS, Chung LP, Lam PW, Tse CW, Wyllie AH and Leung SY . (2002). _Oncogene_, 21, 7585–7592. * Zebedee Z and Hara E . (2001).
_Oncogene_, 20, 8317–8325. Download references ACKNOWLEDGEMENTS This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region (HKU 7006/99M and
7264/01M) and the HM Lui Foundation. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Agnes Sze Wah Chan,
Wai Yin Tsui, Tsun Leung Chan, Annie Shuk Yee Chan, Siu Tsan Yuen & Suet Yi Leung * Department of Surgery, Stanford University School of Medicine, Stanford, 94305, CA, USA Xin Chen, Rui
Li & Samuel So * Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Hong Kong Kent Man Chu Authors * Agnes Sze Wah Chan View author publications You can also search
for this author inPubMed Google Scholar * Wai Yin Tsui View author publications You can also search for this author inPubMed Google Scholar * Xin Chen View author publications You can also
search for this author inPubMed Google Scholar * Kent Man Chu View author publications You can also search for this author inPubMed Google Scholar * Tsun Leung Chan View author publications
You can also search for this author inPubMed Google Scholar * Annie Shuk Yee Chan View author publications You can also search for this author inPubMed Google Scholar * Rui Li View author
publications You can also search for this author inPubMed Google Scholar * Samuel So View author publications You can also search for this author inPubMed Google Scholar * Siu Tsan Yuen View
author publications You can also search for this author inPubMed Google Scholar * Suet Yi Leung View author publications You can also search for this author inPubMed Google Scholar
CORRESPONDING AUTHORS Correspondence to Siu Tsan Yuen or Suet Yi Leung. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Chan, A., Tsui, W., Chen, X. _et
al._ Downregulation of _ID4_ by promoter hypermethylation in gastric adenocarcinoma. _Oncogene_ 22, 6946–6953 (2003). https://doi.org/10.1038/sj.onc.1206799 Download citation * Received: 07
March 2003 * Revised: 20 May 2003 * Accepted: 21 May 2003 * Published: 09 October 2003 * Issue Date: 09 October 2003 * DOI: https://doi.org/10.1038/sj.onc.1206799 SHARE THIS ARTICLE Anyone
you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the
Springer Nature SharedIt content-sharing initiative KEYWORDS * _ID4_ * _hMLH1_ * microarray * promoter methylation * gastric adenocarcinoma
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